Coding

Part:BBa_K130001:Design

Designed by: Basudeb Bhattacharyya   Group: iGEM08_Wisconsin   (2008-10-28)

lipD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 790
    Illegal PstI site found at 868
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 790
    Illegal PstI site found at 868
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 790
    Illegal PstI site found at 868
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 790
    Illegal PstI site found at 868
    Illegal NgoMIV site found at 997
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 511
    Illegal BsaI.rc site found at 319


Design Notes

ORF includes start and stop codon.

PstI present in ORF (two cut sites)

Source

Phanerochaete chrysosporium cDNA library (source: Dr. Dan Cullen, UW-Madison)

References